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Recent Publications by IM Students and Faculty - May 2021

Below are recent publications by IM students, faculty, and affiliates. Would you like your research showcased? Send your paper to in time for the next edition!

Bold Indicates ISU faculty and affiliates 

^Indicates publications by IM students

1. ^Clampitt JM, Madsen ML, Minion FC. Construction of Mycoplasma hyopneumoniaeP97 Null Mutants. Front Microbiol. 2021 Apr 22;12:518791. doi: 10.3389/fmicb.2021.518791. PMID: 33967967; PMCID: PMC8101707.

Abstract: Mycoplasma hyopneumoniae is the causative agent of enzootic pneumonia, a world-wide problem in the pig industry. This disease is characterized by a dry, non-productive cough, labored breathing, and pneumonia. Despite years of research, vaccines are marginally effective, and none fully protect pigs in a production environment. A better understanding of the host-pathogen interactions of the M. hyopneumoniae-pig disease, which are complex and involve both host and pathogen components, is required. Among the surface proteins involved in virulence are members of two gene families called P97 and P102. These proteins are the adhesins directing attachment of the organism to the swine respiratory epithelium. P97 is the major ciliary binding adhesin and has been studied extensively. Monoclonal antibodies that block its binding to swine cilia have contributed extensively to its characterization. In this study we use recombination to construct null mutants of P97 in M. hyopneumoniae and characterize the resulting mutants in terms of loss of protein by immunoblot using monoclonal antibodies, ability to bind purified swine cilia, and adherence to PK15 cells. Various approaches to recombination with this fastidious mycoplasma were tested including intact plasmid DNA, single-stranded DNA, and linear DNA with and without a heterologous RecA protein. Our results indicate that recombination can be used to generate site-specific mutants in M. hyopneumoniae. P97 mutants are deficient in cilia binding and PK15 cell adherence, and lack the characteristic banding pattern seen in immunoblots developed with the anti-P97 monoclonal antibody.

2. Boury, N., Alvarez, K. S., Costas, A. G., Knapp, G. S., & Seipelt-Thiemann, R. L. (2021). Teaching in the Time of COVID-19: Creation of a Digital Internship to Develop Scientific Thinking Skills and Create Science Literacy Exercises for Use in Remote Classrooms. Journal of microbiology & biology education, 22(1).

Importance of the article: Provides resource for faculty to host a digital internship focused on scientific thinking skills.  Specifically, formulating testable hypotheses, designing experiments, mathematical reasoning, communication to a variety of audiences, and impacts of science on society and vice versa.